Table 2 List of secondary antibodies used for immunoblotting (IB), immunohistochemistry (IHC) and immunocytochemistry (ICC)
Secondary antibodies(conjugation) | Host | Company | Catalog # | Application | Folds |
|---|---|---|---|---|---|
Mouse IgG (HRP) | Sheep | GE Healthcare Bioscience (Little Chalfont, UK) | NA931 | WB | 2,000 |
Rabbit IgG (HRP) | Donkey | GE Healthcare Bioscience (Little Chalfont, UK) | NA934 | WB | 4,000 |
Rat IgG (HRP) | Goat | GE Healthcare Bioscience (Little Chalfont, UK) | NA935 | WB | 5,000 |
Rabbit IgG (biotinylated) | Goat | Santa Cruz Biotechnology (Santa Cruz, CA) | SC-2040 | IHC | 200 |
Mouse IgG (Alexa 488 or 546) | Goat | Molecular Probes (Eugene, OR) | A11029 or A11030 | IHC/ICC | 400 |
Rabbit IgG (Alexa 488 or 546) | Goat | Molecular Probes (Eugene, OR) | A11034 or A11035 | IHC | 400 |
Goat IgG (Alexa 488) | Donkey | Molecular Probes (Eugene, OR) | A11055 | IHC | 400 |
Rat IgG (Alexa 488 or 546) | Goat | Molecular Probes (Eugene, OR) | A11006 or A11081 | IHC/ICC | 400 |