Effects of FK866 on NAMPT and PAR formation. NAMPT and PAR formation was evaluated by immunohistochemical analysis in the spinal cord section at 24 h after SCI. Spinal cord section obtained from SCI animals demonstrated positive staining for NAMPT in the white and gray matter (b, see the particular b1). FK866 treatment reduced the degree for positive staining for NAMPT in the spinal cord tissue (c). Moreover, immunoistrochemistry for PAR revealed the occurrence of positive staining for PAR in mice subject to SCI (e). FK866 treatment reduced the degree for positive staining for PAR in the spinal cord tissue (f). This figure is representative of at least 3 experiments performed on different experimental days.