Effects of FK866 on Bax expression. By western blot and immunohistochemical analysis Bax levels were evaluated. Spinal cord obtained from SCI-operated mice exhibited appreciably increased Bax levels (a, a1 and c). FK866 (a, a1 and d) treatment prevented the SCI-induced Bax expression. The relative expression of the protein bands was standardized for densitometric analysis to β-actin levels, reported in panel a, a1 and are expressed as mean ± S.E.M from n = 5/6 spinal cord for each group. **p < 0.001 vs sham, °°p < 0.001 vs SCI + vehicle.