Figure 6
STAT1 expression is detected mainly in IFNγR+ cells. Panels show areas of inflammatory foci or normal appearing white/gray matter (NAW/GM) areas away from the site of inflammation. Infiltrating cells are shown in red (CD45.2 for A-D and M-P or CD45.1 for E-L). IFNγRKO-chimeras expressed CD45.2 both in the CNS and periphery, and STAT1 was rarely detected in isolated cells within infiltrating foci (A, C) or in the normal appearing white/gray matter (NAW/GM) areas away from the site of inflammation (B, D). IFNγRCNSKO mice exhibited STAT1 expression in CD45.1+(IFNγR+) infiltrating cells (E, G) and not in the NAW/GM (F, H). WT-chimeras expressed STAT1 both in CD45.1+ (IFNγR+) infiltrating cells and CD45.1-(IFNγR+) resident cells (I-L), while in IFNγRperiKO mice, STAT1 expression was mainly absent from CD45.2+(IFNγR-) infiltrating cells (M, O) but was localized in CD45.2-(IFNγR+) resident cells in NAW/GM (N, P). Space bar = 50 μm. Inserts show selected magnified fields. Space bar = 10 μm. Nuclei were visualized by DAPI staining. Two (out of 4-6) representative mice per set are shown.