Figure 2

Trichostatin A (TSA) dose-dependently affects temperature and weight gain and increases histone acetylation in female, but not in male, neonatal mice.Data shown were gathered at 14 hours after lipopolysaccharide (LPS) treatment with or without TSA. (A) Mean rectal temperature (n = 6 to 10 pups). (B) Mean change in body weight (n = 6 to 10 pups). (C) Mean acetylated histone-4 expression (Ac-H4)normalised to reference protein histone-2B (H2B) (each n = 14). *P <0.05 treatment effect by one-way analysis of variance (ANOVA), #P <0.05 by post hoc t-test. (D) and (E) Sex-specific Western blot analysis of Ac-H4 (molecular weight 10 kDa) and H2B (molecular weight 15 kDa) showing the effects of LPS (L) and LPS + TSA treatment (+T) in females and males (D) and mean normalised acetylation for female and male mice at 14 hours after intraperitoneal injection (all n = 7). * P<0.05 by Student’s t-test.