Figure 3

B. burgdorferi induces apoptosis in MO3.13 oligodendrocytes in a dose-dependent manner. Apoptosis detected by the in situ TUNEL assay (green) in MBP-stained differentiated MO3.13 cells (red), in medium control (A) and after incubation with live B. burgdorferi for 48 h at multiplicity of infection (MOI) of 10:1 (B), 100:1 (C), and 500:1 (D), as visualized by confocal microscopy. (E) : Graphical representation of the percent apoptosis as detected by the in situ TUNEL assay in differentiated MO3.13 cells held in medium control and after incubation with live B. burgdorferi at MOIs of 10:1, 100:1, and 500:1, respectively, for 48 h (*P < 0.05, **P < 0.01, ***P < 0.001).