Dexamethasone protects differentiated human oligodendrocytes from
-induced apoptosis in a dose dependent fashion. A graphical representation of the percent apoptosis as evaluated by the in situ TUNEL assay in differentiated MO3.13 oligodendrocytes (A) in the presence and absence of dexamethasone (Dex) at 5 μM, 15 μM, and 150 μM concentrations. Confocal images showing apoptosis as observed by the in situ TUNEL assay in differentiated human oligodendrocyte precursor cells (HOPC) incubated for 48 h in medium (B), B. burgdorferi at MOI of 10:1 (C) and B. burgdorferi (10:1) in the presence of dexamethasone at 5 μM (D). The TUNEL signal is seen in green in differentiated HOPC showing expression of myelin basic protein (MBP), in red. (E) Graphical representation of the protective effect of dexamethasone on B. burgdorferi-induced apoptosis in HOPC at 5 μM,15 μM, and 150 μM concentrations, (* P < 0.05).