PrP106-126-stimulated release of interleukin (IL)-1β is dependent on the NACHT, LRR and PYD domains-containing protein (NALP)3 inflammasome. BV2 microglia cells were transfected with either control non-targeting small interfering (si) RNA (N-i), NALP3-targeting siRNA (NALP3-i), or ASC-targeting siRNA (ASC-i). (a) Quantitative PCR analysis of the mRNA expression of NALP3 and ASC in BV2 microglia after siRNA transfection. (b) Western blot analysis of the protein expression of NALP3 and ASC in BV2 microglia after siRNA transfection. (c) ELISA analysis of IL-1β released in the supernatants of BV2 microglia with NALP3 or ASC knock-down treated with PrP106-126. Data represent the mean ± SD of triplicate samples from one of three independent experiments. *P < 0.05, significantly different from control under the same experimental conditions.