Release of interleukin-6 and keratinocyte-derived chemokine from the spleen after middle cerebral artery occlusion. Concentrations of IL-6 and KC were measured in the supernatants of spleen slice cultures and normalized to total protein concentrations. The graphs show the square root (SQRT) of (A) IL-6 and (B) KC concentrations in pg/mg at different time points from control (n = 7), sham (n = 4 to 8), MCAO+vehicle (n = 6 to 10) and MCAO+D-JNKI1 (n = 5 to 8) mice. IL-6 release from the spleen increased slightly, though not significantly, at 4 h in both MCAO+vehicle and MCAO+D-JNKI1 mice. Overall, there is no significant change in IL-6 and KC in spleen supernatants in any of the four groups tested. Results are represented as mean ± SEM.