Figure 2

Comparison of immune response-associated soluble factors concentrations between brain homogenates and maternal serum in poly(I:C) treated animals 6 h after injection. Values were normalized to total protein content of each tissue. (A) Pro- and anti-inflammatory cytokines. IL-1β concentration was significantly lower in maternal serum compared with fetal brain homogenates, while IL-13 and IL-10 were significantly higher. IL-1α, IL-2, IL-3, IL-4, IL-5, IL-6, IL-12(p40), IL-12(p70), IL-15, IL-17, IFN-γ, TNF-α and LIF were not detected in fetal brain. (B) Chemokines. Maternal serum showed significantly higher concentration levels of eotaxin, MCP-1, IP-10 and MIG. MIP-1β, RANTES, KC, LIX and MIP-2 were not detected in fetal brain. (C) CSF. Only VEGF was detected in both tissues but no significant differences in their concentration levels were observed. *P <0.05 based on Student’s t-test comparisons between tissues. Values are expressed as the mean ± standard error of the mean; fetal brain, n = 10; maternal serum, n = 3. ND, not detected.