Analysis of the effect of poly(I:C) treatment on immune response-associated soluble factors expression levels between gestational day 17 and postnatal day 5 brain homogenates. (A) Comparison of IRSF concentrations normalized to total protein content. A significantly lower concentration of the cytokine IL-1β was detected in postnatal brain while IL-13 and IL-15 were significantly higher. The chemokine MIP-1α and the CSF VEGF were reduced in postnatal brain while IP-10, KC, MIG, GM-CSF and M-CSF were significantly higher. (B) Comparison of the percentage change in IRSF concentration levels induced by poly(I:C) between pre- and postnatal brain homogenates 24 h after treatment. Note the higher percentage changes in chemokines and CSF in the postnatal brain compared with less pronounced differences in cytokines. Values are mean ± standard error of the mean. *P <0.05 based on Student’s t-test comparisons. GD17, n = 9; PND5, n = 14. ND, not detected.