Figure 5

Substania nigra pars compacta (SNc) sections were stained with anti-gp91 ^PHOX as a marker for signs of oxidative stress. In agreement with the CD11b ratings, poly(I:C) priming followed by later paraquat treatment resulted in significantly increased expression of gp91^PHOX immunostaining relative to saline-treated controls. (A) Representative photomicrographs showing the degree of gp91^PHOX immunoreactivity in paraquat, poly(I:C), and the combination treatment groups, (note that the poly(I:C)-PQ image was taken from the group that received paraquat 2 days after poly(I:C) pre-treatment), (B) Quantification of ratings (arbitrary scale units) of gp91^PHOX immunoreactivity in the SNc. * P < 0.05, relative to saline-treated controls. Data expressed as mean ± SEM, n = 5–8.