Table 1 Stressors utilized in the chronic unpredictable stress (CUS) group.
Stressor | Description | Duration | |
|---|---|---|---|
Predatory stress | see PS procedure in methods | 30 min | |
Restraint | Consisted of placing each mouse in a 50 mL conical with ample ventilating holes to allow for heat exchange. The mouse was confined but in no way compressed and was able to move its body. | 120 min | |
Restraint + Shaking | Consisted of placing each mouse into a well-ventilated 50 mL conical and resting that restraint tube onto a random orbital shaker at a speed of 1.5 revolutions per second. | 60 min | |
AM Stressors | Fox odor | A single mouse was placed into an empty cage without bedding that contains a piece of filter paper (9 cm in diameter) with 0.2 mL of Fox odor (2, 5-Dihydro-2, 4, 5- trimethylthiazoline; 0.1% v/v) for 15 min. | 15 min |
Novel environment | Mice were placed into 1 of 2 plastic tubs (30 in × 30 in) without bedding | 30 min | |
Multiple cage change | Consisted of replacing each mouse cage with a new cage every 30 min for 4 hours. | 4 hrs | |
Slanted cage | Each mouse cage was tilted to a 45 degree angle | overnight (12 hrs) | |
PM Stressors | Continuous light | Mice were exposed to continuous light | 36 hrs |
Dirty rat bedding | 1/2 of bedding from mouse cage was removed and replaced with soiled rat bedding | overnight (12 hrs) | |
No bedding | Mouse bedding was removed from the home cage | Overnight (12 hrs) |