Figure 3

Histamine-induced motility requires α5β1 integrin involvement. (A) α5β1 integrin is expressed (in green) in the presence of LPS (100 ng/ml) or histamine treatment (100 μM) observed by confocal microscopy. Nuclear labeling (in blue) was obtained with Hoechst 33342 staining. (B) Blockade of α5β1 integrin (α5β1 Ab neutralizing antibody: 10 μg/ml) impeded histamine-stimulated motility. (C) Representative photomicrographs depict the migratory effect induced by histamine, which was abolished when α5β1 integrin was blocked. Data are expressed as mean ± SEM (n = 3-20) and as mean total number of cells migrating across the scratch wound (**p < 0.01, ***p < 0.001, using Bonferroni’s multiple comparison test). Scale bar, 10 μm.