Figure 4
Histamine-induced migration requires p38 and Akt signaling pathways. (A) In scratch wound assays, both histamine- and H4R agonist-induced migration were inhibited in the presence of Akt inhibitor, wortmannin (50 nM) or p38 inhibitor, SB239063 (20 μM). (B) Representative photomicrographs depict the inhibitory effect on migration induced by p38 and Akt signaling blockade. Data are expressed as mean ± SEM (n = 3-20) and as mean total number of cells migrating across the scratch wound (**p < 0.01, ***p < 0.001, using Bonferroni’s multiple comparison test).