LPS-induced motility is inhibited by histamine via
receptor activation. (A) Upon an inflammatory challenge triggered by LPS (100 ng/ml), 100 μM histamine inhibited migration, an effect involving H4R activation. In the presence of histamine, only when H4R was blocked (Ant H4R: JNJ7777120, 5 μM), LPS-induced migration was restored. (B) Representative photomicrographs depict the inhibitory effect of histamine on LPS-induced motility upon H4R activation. Data are expressed as mean ± SEM (n = 3-20) and as mean total number of cells migrating across the scratch wound (*p < 0.05, **p < 0.01, ***p < 0.001, using Bonferroni’s multiple comparison test).