Figure 10

Retinal whole-mount. Double immunostaining for GFAP (in red) and MHC-II (in green) after 15 days of laser-induced OHT. The pressure exerted by the cover glass on the retinal whole-mount, produced a retinal-like section effect in some retinal borders. A-C: in contralateral eyes (A), the GFAP immunoreaction (IR) of Müller cells was weaker than in OHT-eyes (D). MHC-II expression was greater in astrocytes (arrow) than in Müller cells (arrowhead) in (B); D-F: in OHT-eyes, MHC-II was upregulated in Müller cells (arrowhead) in (E), the intensity of the staining being greater than in contralateral eyes (arrowhead) in (B). GFAP-IR was absent or faint in those Müller cells exhibiting MHC-II immunostaining (D-F). Both in contralateral (B) and OHT-eyes (E), the retinal-like section effect enabled us to determine that MHC-II upregulation in Müller cells was preferentially located in the end-foot of the cells. Fluorescence microscopy and image acquisition using the ApoTome. GFAP, glial fibrillary acidic protein; MHC, major histocompatibility complex; OHT, ocular hypertension.