CMV infection of blood–brain barrier cellular components. Human brain cortical astrocytes, BMVEC and pericytes representing the major cellular components of the blood–brain barrier were infected with SBCMV. Cells cultivated to 50% confluence were stained with representative markers. (A) Astrocytes were stained for glial fibillary acidic protein (GFAP), BMVEC for von Willebrand factor (vWF) and brain pericytes with NG2 proteoglycan antibodies. (B) All three cell types were infected with SBCMV for 72 hours. BMVEC, brain microvascular endothelial cells; GFAP, glial fibrillary acidic protein; NG2, neuron-glial antigen 2; SBCMV, primary HCMV isolate from a patient; vWF, von Willebrand factor.