Figure 8From: Infection and upregulation of proinflammatory cytokines in human brain vascular pericytes by human cytomegalovirus Immunohistochemical staining of HIV and CMV coinfected brain tissue. IHC staining of archival human hippocampus brain tissue coinfected with HCMV from an HIV-infected patient. (A/B) Brain tissue IHC-stained for HIV Gag) using True Blue (Kirkegaard & Perry Laboratories, Gaithersburg, MD, USA) as a substrate for immunoperoxidase. Photographs in A and B were taken at 200x and 400x magnification, respectively. (D/C) IHC-stained brain tissue for the HCMV MIE gene protein using True Blue as an immunoperoxidase substrate. Photographs were at 200x and 400x magnification. (E) Brain tissue IHC dual-stained for the pericyte marker NG2 proteogylcan using 3,3-diaminobenzidine (DAB) staining cells brown and CMV MIE gene protein using Vector Red (Vector Laboratories, Burlingame, CA, USA) as a substrate for alkaline phosphatase (dual-stained indicated by arrows). This photograph was taken at 200x magnification. (F) Brain tissue dual-stained by IHC for NG2 proteoglycan and HCMV MIE gene protein showing a cell with a brown-colored cytoplasm and a red nucleus. This image was taken at 600x magnification. All images were taken with a Nikon TE2000S microscope fitted with a CCD camera (Nikon, Tokyo, Japan). CMV, cytomegalovirus; DAB, 3,3-diaminobenzidine; IHC, immunohistochemistry; HCMV, human cytomegalovirus; MIE, major immediate early protein; NG2, neuron-glial antigen 2.Back to article page