Figure 5

The expression of Cat C mRNA was induced following LPS, IL-1β and IL-6 treatments in BV-2 cells. The levels of Cat C mRNA were measured by RT-PCR after treatments of LPS (0 to 100 ng/mL), IL-1β (0 to 10 ng/mL) and IL-6 (0 to 10 ng/mL). The example of PCR product band and averaged gray ratio at the each of treatments were shown in (A) and (D), (B) and (E), (C) and (F), respectively. Note a dose-dependent expression of Cat C mRNA was found in three groups of treatments. *: P < 0.05, **: P < 0.01, compared with untreated control. Data were expressed as mean + SEM from three independent experiments. Cat C, cathepsin C; LPS, lipopolysaccharide; RT-PCR, reverse transcription polymerase chain reaction.