Expression of the α7-nicotinic acetylcholine receptor (α7-nAChR) subunit expression in primary astrocyte cell cultures. (A) Glial fibrillary acidic protein (GFAP) immunofluorescence staining of astrocytes in primary cultured astrocyte cells from the midbrain of C57BL/6 black newborn mice 1–2 days after birth. Scale bar: 50 μm. (B) Reverse transcription (RT)-PCR analysis of α7 subunit mRNA expression in primary cultured astrocyte cells. (C) Western blot of α7 nAChR protein in primary cultured astrocyte cells. (D) Primary mouse astrocytes stained with Alexa Fluor 488 conjugate-labeled α-bungarotoxin and viewed under fluorescence confocal microscopy. Scale bar: 50 μm.