Skip to main content
Figure 7 | Journal of Neuroinflammation

Figure 7

From: α7 nicotinic acetylcholine receptor-mediated neuroprotection against dopaminergic neuron loss in an MPTP mouse model via inhibition of astrocyte activation

Figure 7

The effects of nicotine on activation of extracellular regulated kinase (Erk)1/2 and p38 mitogen-activated protein kinase (MAPK) induced by 1-methyl-4-phenylpyridinium ion (MPP + ) in astrocytes. MPP+ increased Erk1/2 and p38 MAPK phosphorylation indicative of their activation. (A,B) Astrocytes were treated with MPP+ 200 μmol/l for various lengths of time as indicated.(C,D) Astrocytes were pretreated with nicotine 10 μmol/l in the absence or presence of MLA 100 nmol/l for 30 minutes and then challenged with MPP+ 200 μmol/l for 30 minutes. After treatment, astrocytes were harvested, and levels of phosphorylated (A,C) Erk1/2 and (B,D) p38 MAPKs were analyzed. Upper panel shows densitometric analysis of the phosphorylated forms of Erk1/2 and p38 MAPK, while the lower panel shows representative blots. Data are presented as the mean ± SEM of four independent experiments. *P < 0.05 and **P < 0.01 versus the control group; # P < 0.05 and ## P < 0.01 versus the MPP+ treatment alone group.

Back to article page