FTY720 treatment affects virus-specific T cell infiltration into the CNS. S1P1 eGFP mice were i.c. infected with JHMV (150 PFU) and treated daily with FTY720 (3 mg/kg) starting on day 5 p.i. (A) Representative flow staining showing intracellular IFN-γ staining by CNS infiltrating T cells following exposure to CD4+ T cell epitope (M133-144) and CD8+ T cell epitope (S510-518) at day 7 p.i. in mice treated with either FTY720 (3 mg/kg) or vehicle control. (B) Reduced frequency of virus-specific IFN-γ producing CD4+ and CD8+ T cells in the CNS following FTY720 treatment at defined times p.i. (C, D) Similar frequencies of infiltrating ASCs (CD45−CD19low CD138+) in the brains of vehicle- and FTY720-treated mice. Data in B and D are presented as average ± SEM and represent a minimum of two independent experiments with at least five mice/experimental group. *P <0.05, *** P <0.001.