FTY720 treatment reduces the severity of JHMV-induced demyelination. (A) Representative LFB and H&E-stained spinal cord images showing an overall reduction in the severity of inflammation and demyelination within white matter tracts (dashed lines) in JHMV-infected eGFP S1P1 mice treated with FTY720 (3 mg/kg) compared to vehicle control-treated mice at day 21 p.i. (B) FTY720 treatment results in reduced neuroinflammation (P < 0.05) and demyelination (P < 0.05) compared to mice treated with the vehicle control. Flow analysis of spinal cords demonstrates reduced entry of both CD8+ (C) and CD4+ T cells (D), while infiltration of virus-specific T cells was not affected. Data in panel B represent two independent experiments with a minimum of ten mice/group. Data in panels C and D are presented as average + SEM and represent two independent experiments with a minimum of five mice/group. *P <0.05. The scale bar in panel A represents 200 μm. SSC, side scatter.