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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Lithium ameliorates lipopolysaccharide-induced microglial activation via inhibition of toll-like receptor 4 expression by activating the PI3K/Akt/FoxO1 pathway

Figure 5

Effects of lithium on PI3K/Akt activation and TLR4 expression in microglia. (a) Representative images of western blot for the activation of PI3K, Akt, FoxO1. The primary microglial cells were incubated with LiCl (3 mM) for 15 minutes; 30 minutes; and 1, 2, 4, 8, 12, and 24 hours. (c) Effects of lithium on TLR4 expression. The cells were incubated with various concentrations of LiCl for 24 hours. The levels of p-PI3K, p-Akt, p-FoxO1 (b) and TLR4 (d) were quantified and normalized with their respective total PI3K, Akt, FoxO1 or GAPDH levels. Each value was then expressed relative to the one treated with medium, which was set as 1. *P <0.05, **P <0.01 versus the response to medium alone. The data are presented as the mean ± s.e.m. of three independent experiments.

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