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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Microglia P2Y6 receptors mediate nitric oxide release and astrocyte apoptosis

Figure 4

Pyrimidine receptors and signaling pathway involved in the inhibition of cell proliferation mediated by uracil nucleotides. (A) Lipopolysaccharide (LPS) cultures were incubated with the nucleotides for 48 h and in the last 24 h methyl-[3H]-thymidine was added to the medium at a concentration of 1 μCi/ml. The P2Y6 antagonist MRS 2578 and enzyme inhibitors were added to the medium 1 h before the nucleotides, except PTX, which was added to the medium 24 h before. Effects in cell proliferation were estimated by methyl-[3H]-thymidine incorporation and expressed as percentage of change from the respective control. Values are means ± SEM from eight to twenty experiments. *P <0.05, significant differences from respective control; +P <0.05, significant differences from the agonist alone. (B) Representative western blots showing the expression of P2Y6 receptors obtained from whole cell lysates. Two bands of 25 kDa, one of 36 kDa and another of 86 kDa, specifically reacted with the anti-P2Y6 antibody. These bands were absent in the presence of the respective neutralizing peptide (np).

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