Cellular distribution and localization of P2Y
receptors in lipopolysaccharide (LPS) cultures. Microglia were labeled with mouse anti-CD11b (Alexa Fluor 488, green), P2Y6 receptors were labeled with rabbit anti-P2Y6 (TRICT, red) and nuclei were labeled with Hoechst 33258 (blue). The orange spots represent the expression of P2Y6 receptors that are coincident with microglia, but not in astrocytes (blue nuclei that do not label with CD11b and P2Y6 receptor antibodies). Scale bar: 20 μM.