Figure 4

Detection of retinal ganglion cell (RGC) phagocytosis by GFP ^hi and GFP ^lo CD11b ⁺ cells in CDG retina post-ONC. (A) Depiction of CD45^hi and CD45^med gating, elimination of 1A8⁺ PMN, and selection for CD11b⁺ cells that are GFP^lo or GFP^hi. (B) Sequential confocal sections of GFP^hi DC engulfing DiI-labeled RGC seven days post-ONC. DiI-labeled RGC, red; GFP^hi DC, green; DAPI-stained nuclei, blue. (C) Detection and quantitation of DiI-labeled CD45^medCD11b⁺GFP^hi DC and GFP^lo cells by flow cytometry. Ipsilateral retinas were labeled by injection of DiI into the superior colliculus. After seven days, mice were given an ONC; retinas were harvested six days later.