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Figure 9 | Journal of Neuroinflammation

Figure 9

From: Retinal dendritic cell recruitment, but not function, was inhibited in MyD88 and TRIF deficient mice

Figure 9

Systemic administration of DTx to the CD11c-DTR/GFP mice leads to depletion of the GFP hi DC. (A-C) Serial fundus photographs of the same retina following an ONC and DTx treatment via ip injection. (A) GFPhi DC at 12 days post-ONC. (B) Fundus photograph of retina one day after ip injection of 200 ng DTx. (C) Photograph of retina two days post-ip DTx injection. (D) Flow cytometry of retina following ip injection of DTx under two different protocols; six daily injections of 10 ng into a naive mouse, and 2 injections of 100 ng into a mouse at days 11 and 12 post-ONC. (E) Daily serial injections of 10 ng DTx in naive mice. (F-K) Confocal microscopy of retinal flatmounts in ONC-injured retinas. (F-H) DTx (200 ng) was injected ip 48 hours prior to harvest, at 15 days post-ONC. (F) Cells and nerve fibers in the NFL post-DTx. (G-H) Cells at two levels in the IPL. All images in each vertical column are from the same confocal field at different depths into the retina. (I-K) Saline, 1 μl, was injected ip 48 hours prior to harvest, at 15 days post-ONC. (I) Cells and nerve fibers in the NFL. (J-K) Cells at two levels in the IPL. GFPloCD11b+ cells stained red; GFPhiCD11b+ cells stained yellow-green. NFL, nerve fiber layer; IPL, inner plexiform layer at two depths is shown, immediately below the retinal ganglion cell layer (RGC) soma, and adjacent to the INL.

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