Figure 1

Rabies virus (RABV) infection induces high mortality and excessive inflammation in the central nervous system of suckling mice. Suckling mice were intracerebrally (i.c.) infected with aG or CTN (5.6 × 10³ FFU), and (A) the survival rates were monitored (n = 10 in each group). (B) The relative mRNA levels of RABV N protein were determined in aG- and CTN-infected mice on day 5 and day 7 postinfection (p.i.). (C) On day 7 p.i., the expression profiles of various inflammatory proteins in brain samples were evaluated by RayBio™ Mouse Inflammation Antibody Array G. (D) The protein levels of inflammatory molecules that were enhanced over 1.5-fold in the brain samples infected with aG and CTN are listed (n = 3). The relative mRNA levels of CCL5, CCL2 (E), IL-6, IL-1β, IL-12, IL-17 (F) in brain samples were verified by quantitative reverse transcriptase PCR (qRT-PCR) after aG and CTN infection. Data were presented as means ± SD (*P <0.05; **P <0.01; ***P <0.001).