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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Met-CCL5 represents an immunotherapy strategy to ameliorate rabies virus infection

Figure 4

Exogenous CCL5 treatment and rabies virus infection enhance neuron apoptosis in the central nervous system. Mice were intracerebrally (i.c.) infected with PBS or exogenous CCL5 (25 μl, 5 μg/ml). (A) On day 1 postinfection (p.i.), single cell suspension was prepared from the brains and stained with propidium iodide (PI) and AnnexinV to analyze cell apoptosis by flow cytometry. (B) Suckling mice infected with aG and CTN (5.6 × 103 FFU) were euthanized, and brains were removed to prepared frozen section on day 5 p.i.. Pathological changes of neurons, including irregular nuclear chromatin condensation, vacuolation, and neuron necrosis in cerebral cortex and hippocampus, were observed by cresyl violet staining (Magnification: 40×). (C and D) Brain sections were subjected to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and the TUNEL-staining positive cells were quantified (n = 3). (E) Cleaved caspase-3 was stained with caspase-3 antibody (red), and the cell nuclei were stained with DAPI (blue). Image magnification: a-f (60×), g-i (20×). Data were presented as means ± SD (**P <0.01; ***P <0.001).

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