Figure 5

Met-CCL5 treatment reduces the central nervous system inflammation after rabies virus infection. Suckling mice were intracerebrally (i.c.) infected with aG (5.6 × 10³ FFU) followed by daily intraperitoneal (i.p.) administration with PBS or Met-CCL5 (100 μg/ml, 100 μl). (A) The survival rates in each group were recorded. (B) On day 5 postinfection (p.i.), the relative mRNA levels of CCL5 (B, left panel) and IL-6 (B, right panel) and RABV N protein (C) in the brains were quantified by quantitative reverse transcriptase PCR (qRT-PCR). (D) Bone marrow-derived macrophage (BMM) were treated with CCL5 (60 ng/ml, 6 hrs), and the relative mRNA levels of CCL5, IL-6 and TNF-α were measured by qRT-PCR. (E) BMMs were stimulated for 6 hrs with poly I:C alone, in the absence or presence of CCL5 or CCL5 and Met-CCL5. The relative mRNA levels of CCL5 and IL-6 were determined using qRT-PCR. Data were presented as means ± SD (*P <0.05; **P <0.01; ***P <0.001).