Figure 6

Met-CCL5 treatment prolongs the survival time in adult mice after rabies virus infection. The 6-week-old ICR mice (n ≥ 3) were intramuscularly (i.m.) infected with HN10 (5.6 × 10³ FFU), and the relative mRNA levels of CCL5, IL-12, CCL3, IL-6 were evaluated by quantitative reverse transcriptase PCR (qRT-PCR) (B). The 6-week-old ICR mice were intracerebrally (i.c.) infected with aG or intramuscularly (i.m.) infected with HN10 followed by intraperitoneal (i.p.) administration of 100 μl Met-CCL5 at a concentration of 200 μg/ml or 20 μg/ml and 100 μl synthesized random disordered Met-CCL5 (200 μg/ml) as negative controls from day 2 postinfection (p.i.). Mice were monitored for 21 days, and the survival rate in the aG-infected group (A) and in the HN10 infected group (C) were recorded (n = 10; *P <0.05; **P <0.01; ***P <0.001). When the negative control mice infected with HN10 showed clinical signs, Met-CCL5 treated mice and control mice were euthanized at the same time, brains were removed, and these brains were subjected to analysis for pro-inflammatory chemokine/cytokine expression and virus load using qRT-PCR (D). Data were presented as means ± SD (*P <0.05; **P <0.01; ***P <0.001).