Figure 2
SCM-198 prevented lipopolysaccharide (LPS)- or Aβ 1-40 -induced morphological alterations in microglia. BV-2 cells or primary microglia were pretreated with indicated concentrations of 1 μM SCM-198(SCM), 100 μM ibuprofen (IBU) or 20 μM donepezil (DON) for 2 hours, then stimulated with 1 μg/ml LPS (a) or 3 μM Aβ1-40 (b) for 24 hours. Morphological changes were quantified by measuring radius ratio (radius ratio = maximum radius/minimum radius) (c-d) (scale bar, 100 μM). Data represent mean ± SEM of more than three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, Tukey’s test versus only LPS- or only Aβ1-40-treated group; #P < 0.05, ##P < 0.01, ###P < 0.001, Tukey’s test versus control group.