SCM-198 could directly protect neurons from Aβ
toxicity or indirectly protect neurons via modulating microglia in microglia/neuron co-culture system. BV-2 cells were pretreated with 0.1 to 10 μM SCM or 100 μM ibuprofen (IBU), and stimulated with 1 μg/ml lipopolysaccharide (LPS) for 2 hours, washed with fresh DMEM medium and co-cultured with cortical neurons for 24 hours. Neuronal viability was measured by 5-diphenyltetrazolium bromide (MTT) assay (a) and phosphorylation of ERK (p-ERK) and tau (p-tau) of neurons were detected by Western blot (b-f). Primary cortical neurons were pretreated with indicated concentrations of SCM or 20 μM donepezil (DON) for 2 hours, followed by 12-hour stimulation of 20 μM Aβ1-40. Neuronal viability (g) and LDH leakage (h) were measure by sulforhodamine B (SRB) assay and the lactate dehydrogenase (LDH) kit. Data represent mean ± SEM of more than three independent experiments. *P < 0.05, **P < 0.01, Tukey’s test versus only LPS- or only Aβ1-40-treated group; #P < 0.05, ##P < 0.01, Tukey’s test versus control group.