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Table 1 Sequence of specific primers used for quantitative real-time reverse transcription PCR

From: Expression of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) and its roles in activated microglia in vivo and in vitro

Gene Sequence

CNPase

Forward

gcaggaggtggtgaagagat

Reverse

cagatggcttgtccagatca

TNF-α

Forward

cgtcagccgatttgctatct

Reverse

cggactccgcaaagtctaag

IL-1β

Forward

gcccatcctctgtgactcat

Reverse

aggccacaggtattttgtcg

iNOS

Forward

gcttgtctctgggtcctctg

Reverse

ctcactgggacagcacagaa

β-actin

Forward

ggattccatacccaagaagga

Reverse

gaagagctatgagctgcctga

  1. CNPase, 2′,3′-cyclic nucleotide 3′-phosphodiesterase; IL-1β: interleukin-1beta; iNOS, inducible nitric oxide synthase; PCR, polymerase chain reaction; TNF-α, tumor necrosis factor alpha.