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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Mesenchymal stem cells exert anti-proliferative effect on lipopolysaccharide-stimulated BV2 microglia by reducing tumour necrosis factor-α levels

Figure 1

Mesenchymal stem cells inhibits BV2 microglia proliferation independent of nitric oxide. (a) BV2 cells and Mesenchymal stem cells (MSC) were seeded at a 1:0.2 ratio in a 96-well plate with 1 μg/ml lipopolysaccharide (LPS) and 1000 μm N-nitro-L-arginine methyl ester (L-NAME) for 48 hours, and BV2 proliferation was determined with a tritiated thymidine (3H-TdR) incorporation assay. Values are expressed as mean ± SD of percentage BV2 proliferation from three independent experiments. (b) NO2 - concentration in culture supernatant was determined using the Griess assay. BV2 cells and MSC were cultured in 12-well plates at a 0.2 ratio (BV2: MSC) and stimulated with 1 μg/ml LPS, and NO2 - was assayed at 48 hours. Values are expressed as mean ± standard deviation of NO2 - in μm from three independent experiments. *P < 0.01, versus BV2 cells; #P < 0.01, versus BV2 +μlPS.

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