Figure 2

Co-culture does not induce apoptosis in BV2 cells and mesenchymal stem cells. Scatter plots show percentage of viable (lower left), early apoptotic (lower right), late apoptotic (upper right) and necrotic (upper left) populations in (a) BV2 cells and (b) mesenchymal stem cells (MSC) as determined by Annexin-V/propidium iodide (PI) staining 48 hours post-lipopolysaccharide (LPS) stimulation. The number within each quadrant of plot indicates percentage of cells. Results are from a representative of three independent experiments.