IL-6 does not play a role in mesenchymal stem cell (MSC)-mediated modulation of nitric oxide and proliferation in BV2:MSC co-cultures. BV2 cells and MSC were seeded at a 1:0.2 ratio into 12-well plates (Griess assay) or 96-well plates (proliferation assay). After overnight incubation, 1 μg/ml lipopolysaccharide (LPS) and interleukin (IL)-6 blocking antibodies at concentrations indicated below the graph were added to co-cultures and (a) proliferation and (b) NO2
- concentration were analysed at 48 hours. Values are expressed as mean ± standard deviation from three independent experiments. Controls are respective cultures without blocking antibodies. *P < 0.05, versus control BV2 cells; #P < 0.05, versus control BV2 +μlPS.