|
Cell cycle distribution (BV2 Microglia: MSC direct coculture)
|
|---|
| |
BV2
|
BV2 +μlPS
|
BV2 +μmSC +μlPS
|
|---|
|
G0/G1
|
S
|
G2/M
|
G0/G1
|
S
|
G2/M
|
G0/G1
|
S
|
G2/M
|
|---|
|
24 hrs
|
42.8 ± 1.5
|
49.3 ± 3.7
|
7.0 ± 2.5
|
34.2 ± 0.6*
|
62.0 ± 3.8#
|
3.8 ± 3.3
|
44.3 ± 1.8†
|
50.1 ± 1.0‡
|
5.6 ± 4.9
|
|
48 hrs
|
50.7 ± 1.9
|
44.2 ± 1.1
|
4.7 ± 1.2
|
38.2 ± 1.2*
|
59.0 ± 2.7#
|
1.8 ± 2.2
|
43.9 ± 1.9†
|
48.6 ± 1.2‡
|
6.3 ± 3.7
|
|
72 hrs
|
77.6 ± 0.9
|
19.3 ± 0.9
|
3.1 ± 0.1
|
48.2 ± 1.5*
|
51.6 ± 1.8#
|
0.3 ± 0.3
|
52.4 ± 5.6
|
41.3 ± 2.4‡
|
1.9 ± 3.0
|
|
96 hrs
|
83.8 ± 4.0
|
14.1 ± 3.7
|
2.1 ± 2.3
|
63.2 ± 1.3*
|
35.7 ± 21.1#
|
1.0 ± 1.1
|
75.6 ± 1.7†
|
18.4 ± 0.9‡
|
6.0 ± 1.1€
|
- BV2 cells and Mesenchymal stem cells (MSC) were seeded at a 1:0.2 ratio in 6-well plates and left overnight prior to lipopolysaccharide (LPS) stimulation (1 μg/ml). Cell cycle analysis of BV2 cells (CD45 + ve population in co-culture) was performed using propidium iodide (PI) staining at the time points indicated. Data show mean percentage population of BV2 cells ± standard deviation from three independent experiments. *P < 0.05, #
P < 0.05, versus respective BV2 alone controls. †P < 0.05, ‡ P < 0.05, versus the corresponding phases in BV2 +μlPS.
