Mesenchymal stem cells exert anti-proliferative effect on lipopolysaccharide-stimulated BV2 microglia by reducing tumour necrosis factor-α levels

Table 1 Co-culture with Mesenchymal stem cells continues to modulate BV2 cell cycle until 96 hours but does not induce a cell cycle arrest

Cell cycle distribution (BV2 Microglia: MSC direct coculture)
	BV2			BV2 +μlPS			BV2 +μmSC +μlPS
	G0/G1	S	G2/M	G0/G1	S	G2/M	G0/G1	S	G2/M
24 hrs	42.8 ± 1.5	49.3 ± 3.7	7.0 ± 2.5	34.2 ± 0.6^*	62.0 ± 3.8^#	3.8 ± 3.3	44.3 ± 1.8^†	50.1 ± 1.0^‡	5.6 ± 4.9
48 hrs	50.7 ± 1.9	44.2 ± 1.1	4.7 ± 1.2	38.2 ± 1.2^*	59.0 ± 2.7^#	1.8 ± 2.2	43.9 ± 1.9^†	48.6 ± 1.2^‡	6.3 ± 3.7
72 hrs	77.6 ± 0.9	19.3 ± 0.9	3.1 ± 0.1	48.2 ± 1.5^*	51.6 ± 1.8^#	0.3 ± 0.3	52.4 ± 5.6	41.3 ± 2.4^‡	1.9 ± 3.0
96 hrs	83.8 ± 4.0	14.1 ± 3.7	2.1 ± 2.3	63.2 ± 1.3^*	35.7 ± 21.1^#	1.0 ± 1.1	75.6 ± 1.7^†	18.4 ± 0.9^‡	6.0 ± 1.1^€

BV2 cells and Mesenchymal stem cells (MSC) were seeded at a 1:0.2 ratio in 6-well plates and left overnight prior to lipopolysaccharide (LPS) stimulation (1 μg/ml). Cell cycle analysis of BV2 cells (CD45 + ve population in co-culture) was performed using propidium iodide (PI) staining at the time points indicated. Data show mean percentage population of BV2 cells ± standard deviation from three independent experiments. *P < 0.05, ^# P < 0.05, versus respective BV2 alone controls. †P < 0.05, ‡ P < 0.05, versus the corresponding phases in BV2 +μlPS.