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Table 1 Co-culture with Mesenchymal stem cells continues to modulate BV2 cell cycle until 96 hours but does not induce a cell cycle arrest

From: Mesenchymal stem cells exert anti-proliferative effect on lipopolysaccharide-stimulated BV2 microglia by reducing tumour necrosis factor-α levels

Cell cycle distribution (BV2 Microglia: MSC direct coculture)
  BV2 BV2 +μlPS BV2 +μmSC +μlPS
G0/G1 S G2/M G0/G1 S G2/M G0/G1 S G2/M
24 hrs 42.8 ± 1.5 49.3 ± 3.7 7.0 ± 2.5 34.2 ± 0.6* 62.0 ± 3.8# 3.8 ± 3.3 44.3 ± 1.8 50.1 ± 1.0 5.6 ± 4.9
48 hrs 50.7 ± 1.9 44.2 ± 1.1 4.7 ± 1.2 38.2 ± 1.2* 59.0 ± 2.7# 1.8 ± 2.2 43.9 ± 1.9 48.6 ± 1.2 6.3 ± 3.7
72 hrs 77.6 ± 0.9 19.3 ± 0.9 3.1 ± 0.1 48.2 ± 1.5* 51.6 ± 1.8# 0.3 ± 0.3 52.4 ± 5.6 41.3 ± 2.4 1.9 ± 3.0
96 hrs 83.8 ± 4.0 14.1 ± 3.7 2.1 ± 2.3 63.2 ± 1.3* 35.7 ± 21.1# 1.0 ± 1.1 75.6 ± 1.7 18.4 ± 0.9 6.0 ± 1.1
  1. BV2 cells and Mesenchymal stem cells (MSC) were seeded at a 1:0.2 ratio in 6-well plates and left overnight prior to lipopolysaccharide (LPS) stimulation (1 μg/ml). Cell cycle analysis of BV2 cells (CD45 + ve population in co-culture) was performed using propidium iodide (PI) staining at the time points indicated. Data show mean percentage population of BV2 cells ± standard deviation from three independent experiments. *P < 0.05, # P < 0.05, versus respective BV2 alone controls. †P < 0.05, ‡ P < 0.05, versus the corresponding phases in BV2 +μlPS.