Figure 1

Structure and purification of recombinant tau proteins followed by the immunization paradigm. (A) Schematic presentation of the sequence and commassie stain of recombinant full length tau protein (wild-type (Wt)-tau, 4R0N) or tau mutated at P301L (P301L-tau, 4R0N). (B) Non-transgenic and rTg4510 mice were immunized three times in alternating weeks, followed by a 10-week resting period, then boosted three times every 3 weeks. After the vaccination period, tissue and blood specimens were analyzed for cellular immune responses and neuropathological changes. Ab, antibody; MW, molecular weight.