Figure 2
Immunizations with Wt-tau and P301L-tau proteins formulated in Quil-A induced robust titers of anti-tau antibody in rTg4510 and littermate mice. Mean endpoint antibody titers specific for wild-type (Wt)-tau (A,D) and P301L-tau (B,E) were evaluated in pooled sera of mice (n = 4 per group) at days 38, 68, and 147. Endpoint titers of antibodies represents the highest sera dilution (1:108,000 from and initial dilution of 1:3,000) with two-fold signal above the cutoff. The cutoff was determined as the titer of pre-immune sera at the same dilution. ELISAs were repeated three times and average ± SD of three ELISAs is presented (student’s t-test was performed, **P < 0.01). (C,F) Individual sera of immunized rTg4510 mice and littermates at day 38 (1:1,000) were analyzed and IgG anti-tau antibody of IgG1, IgG2ab, and IgG2b isotypes were detected. Error bars indicate the average ± SD (n = 4).