Figure 4

Immunogenicity of tau protein detected by spot array. (A) The profile of the epitope mapping experiment where the fluorescence intensities at a wavelength of 532 nm, after background subtraction, for each animal group tested in duplicate is shown. The domain organization of human tau in the central nervous system (441 residues, htau40) containing the I1-I2 inserts on the N-terminal and the R1-R4 repeats is shown. The domain boundaries are labeled by the residue numbers. Five peaks displayed the highest fluorescence intensity (peak 1-5). (B) Amino acid sequence of 4R2N expressed in the central nervous system. Blue colored amino acids represent the five peptide sequences with the highest immunogenicity identified in the spot array. Each highlighted peptide sequence corresponds to the peak 1-5 in (A). Average fluorescence intensity ± SEM is shown for each sequence. a.u., Area under; Tg, transgenic; WT, wild-type.