Figure 5

Reduced activated microglia burden following immunization with wild-type (WT)- or P301L-tau proteins. (A) Representative micrographs of brain regions (anterior cortex (ACX), hippocampus (HPC), and entorhinal cortex (ECX)) immunostained for CD45 microglia marker. (B) Percent positive area analysis of the brain regions demonstrated significant reductions in CD45 microglia burden in both Wt-tau and P301L-tau immunized rTg4510 animals. Immunized non-transgenic animals displayed low levels of activated microglia, hence pooled values were graphed. One way analysis of variance, * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar is 200 μm, inset 20 μm. NTG, non-transgenic; PBS, phosphate-buffered saline.