Figure 6

Reduced inflammation following vaccination with wild-type (Wt)- or P301L-tau proteins. (A) Representative micrographs of hippocampal region positive stain for glial fibrillary acidic protein (GFAP; green) and CD11b (red) in immunized rTg4510 and non-transgenic (NTG) littermates. (B,C). Quantification of percent positive area of hippocampus demonstrated significant reduction in the levels of GFAP following P301L-tau but not Wt-tau immunization. No significant reduction in CD11b immunoreactivity was observed following rTg4510 mice immunization compare to phosphate-buffered saline (PBS)-injected littermates. Immunized non-transgenic animals displayed low levels of GFAP and CD11b immunoreactivity (pooled values). Values are normalized to the PBS control group and student’s t-test was performed, **P < 0.01. Scale bar is 200 μm for regions, 20 μm for insets.