Epitope analysis following active immunization with tau proteins reveals immunogens implicated in tau pathogenesis

Table 2 Immunohistochemical changes measured in immunized mice

	Brain % positive area (average ± SEM)	P-values (Studentt-test)
H150 (total tau)
Wt-tau	0.68 ± 0.03*	0.0179*
P301L tau	0.87 ± 0.17	0.6452
AT8 (pS202-Thr205)
Wt-tau	0.58 ± 0.05*	0.0295*
P301L tau	0.55 ± 0.03*	0.0377*
pS199/202
Wt-tau	0.59 ± 0.04	0.0721
P301L tau	1.29 ± 0.19	0.3600
pS396
Wt-tau	0.96 ± 0.06	0.8089
P301L tau	0.99 ± 0.04	0.9700
Gallyas
Wt-tau	1.27 ± 0.06	0.4596
P301L tau	0.81 ± 0.06	0.5839
CD45
Wt-tau	0.4 ± 0.03	0.0001*
P301L tau	0.51 ± 0.03	0.0001*
CD11b
Wt-tau	0.12 ± 0.07	0.1202
P301L tau	0.04 ± 0.02	0.0550
GFAP (HPC)
Wt-tau	0.85 ± 0.1	0.2977
P301L tau	0.7 ± 0.05	0.0066*

The ratio of percent positive area values for each immunized group to the phosphate-buffered saline (PBS)-injected mice is presented for total tau, p-tau and inflammatory markers (average ± SEM). Values in bold and asterisk indicate markers that are significantly reduced by immunization. Statistical analyses were performed by student’s t-test, *P < 0.05. GFAP, glial fibrillary acidic protein; HPC, hippocampus; Wt, wild-type.

ISSN: 1742-2094