Figure 2
From: Plasminogen in cerebrospinal fluid originates from circulating blood
Determination of plasminogen in rat plasma, cerebrospinal fluid and urine. Samples were obtained from rats challenged with saline (group C1) versus rats challenged with lipopolysaccharide (LPS) (group L1) (see flowchart in Figure 1). (A) Representative immunoblot obtained for plasminogen detection using a rabbit antibody to mouse plasminogen. Electrophoresis of reference rat plasminogen (Pg) in an equal volume (10 μl) of plasma diluted 1:50, cerebrospinal fluid (CSF) and urine. (B) Box-and-whisker plot of plasminogen in plasma, CSF and urine after immunoblot and densitometric analyses. Results represent median (25th to 75th interquartile range (IQR)). *P = 0.0002 and §P = 0.0071 -Whitney U test), respectively. (C) Box-and-whisker plot of measurement of the activation of plasminogen in CSF samples (chromogenic substrate assay). Results represent median (IQR) of the velocity of plasmin formation. #P = 0.0045 (Mann-Whitney U test). mOD: milli optical density.