Figure 6

Ionized calcium-binding adapter molecule 1 protein expression 6 days and 21 days after partial sciatic nerve ligation. Immunohistological staining and quantification of the microglial marker ionized calcium-binding adapter molecule 1 (Iba1) at 6 days (A-E) and at 21 days (A-E’) on the ipsilateral side of the lumbar spinal cord with low and high magnifications. Iba1 staining of sham-operated animals either vehicle-injected (A,A’) or MSC-injected (B,B’) and PSNL animals with vehicle (C,C’) or MSCs (D,D’). Scale bar represents 500 μM. (E,E’) Quantification of the mean grey intensity within the total dorsal horn with SEM, two-way analysis of variance and Tukey post-hoc test for statistical analysis, *P < 0.05 and **P < 0.01. Groups at 6 days after PSNL consisted of 3 to 4 animals and groups at 21 days after PSNL consisted of 5 to 7 animals.