Skip to main content
Figure 9 | Journal of Neuroinflammation

Figure 9

From: Neuronal uptake of anti-Hu antibody, but not anti-Ri antibody, leads to cell death in brain slice cultures

Figure 9

Anti-Ri antibody did not produce neuronal death despite prolonged incubation. Cultures of rat cerebellum were incubated with anti-Ri or control antibody and followed for 219 hours (“Control” is shown at 219 hours). Images shown demonstrate slice cultures imaged at each time point at 20× (upper panels) and 60× (lower panels) magnification. Although antibody uptake occurred in neurons throughout the cerebellum, staining by SYTOX dyes, indicating cell death, and fluorochrome-labeled inhibitors of caspases (FLICA), indicating apoptosis, did not exceed that seen in control cultures at the same time points. A similar lack of cytotoxicity over this same period was observed in hippocampal slice cultures incubated with anti-Ri antibody (see Additional file 4). Images are representative of studies employing anti-Ri sera from both patients, carried out in triplicate. Magnification bar = 20 μm.

Back to article page