Figure 2

Comparative immunofluorescent analysis uncovered the higher potency of TIB67 macrophages phagocytose tau oligomers comparing to BV2 microglia. Live phagocytes were labelled with MitoTracker ™ Green FM green-fluorescent mitochondrial stain and then challenged with red fluorescent latex beads or with oligomeric tau labelled with red Alexa Fluor 546. The panels show quantification data of TIB67 macrophages (A), BV2 microglia (B) engulfing red fluorescent beads in the presence or absence of LPS treatment. (C) Quantitative comparison of TIB67 and BV2 phagocytosis of latex beads after 24 hours. Quantification of TIB67 macrophages (D) and BV2 microglia (E) phagocytosing tau oligomers in the presence or absence of LPS treatment. (F) Quantitative comparison of TIB67 and BV2 primary microglia cell phagocytosis of tau oligomers after 24 hours. The experiments were run in triplicates. Data are presented as mean ± standard error of the mean (SEM). Statistical significance was defined as P <0.05 (*) or P <0.01 (**). Abbreviation: P, p-value.